Measurement

Part:BBa_J119023:Design

Designed by: Samantha Huckuntod   Group: Eckdahl Lab   (2011-12-14)


RFP GGA BD18 destination vector - BbsI and BsaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 924
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 925
    Illegal PstI site found at 939
    Illegal NotI site found at 7
    Illegal NotI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 925
    Illegal PstI site found at 939
    Illegal AgeI site found at 797
    Illegal AgeI site found at 909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 135
    Illegal BsaI.rc site found at 34


Design Notes

Golden Gate Assembly, replacement of TT with promoter, BD18 bicistronic translational junction engineered by Vivek Mutalik and The BIOFAB Team at biofab.org.


Source

Synthetic oligos cloned into existing part.

References